![]() ![]() Hermanson GT (ed) (1995) Bioconjugate Techniques. (FNAB) and hydroxyl group of the cellulose in an alkaline medium. Sabanayagam CR, Smith CL, Cantor CR (2000) Nucleic Acids Res 28:e33, ii–iv The enzyme, immobilized through the photolinker, is found to give a twofold increase. Material Safety Data Sheet or SDS for Hydroxyethyl photolinker 851049 from Merck for download or viewing in the browser. Reichmuth P, Sigrist H, Badertscher M, Morf WE, de Rooij NF, Pretsch E (2002) Bioconjug Chem 13:90–96 Safety Data Sheet for Hydroxyethyl photolinker 851049. Sigrist H, Collioud A, Clemence J-F, Gao H, Luginbuehl R, Saenger M, Sundarababu G (1995) Opt Eng (Bellingham, Washington) 34:2339–2348 Haynes CA, Norde W (1995) J Colloid Interface Sci 169:313–328īuijs J, Norde W, Lichtenbelt JWT (1996) Langmuir 12:1605–1613Ĭhevrier D, Rasmussen SR, Guesdon J (1993) Mol Cell Probes 7:187–197 The generator can be used to: Remove the smell of cigarette smoke from a room. ![]() You simply need to plug in and turn on the device, and it will go to work immediately, cleaning the air. Ratner BD (1995) Biosens Bioelectron 10:797–804īurke CS, Polerecky L, MacCraith BD (2004) Meas Sci Technol 15:1140–1145 A hydroxyl generator is a device that uses UV-C to remove odors, volatile organic compounds (VOCs), virus particles, and bacteria particles from the air. The following reactions were performed to protect the free hydroxyl group on the. Wessa T, Rapp M, Sigrist H (1999) Colloids Surf B Biointerfaces 15:139–146 Due to the poor solubility, the synthesis of the tag-photolinker. For this study DNA oligonucleotides were chosen as a model system of biomolecular probes, and fluorescence detection of DNA microarrays served as method of detection. Of all photolinkers and substrates tested, PTD as photolinker and COC as substrate showed the highest photolinking efficiencies and fastest reaction times. We compared the overall photolinking efficiency of all photolinkers with respect to the polymer substrate they are applied to, and we found considerable differences for certain photolinker/substrate combinations. The influence of substrate material is discussed, and three different polymers served as representative substrates: poly(methyl methacrylate) (PMMA), polystyrene (PS), and a cycloolefin copolymer (COC). The influence of these variables are investigated for four prominent photolinkers: ketyl-reactive benzophenone (BP) and anthraquinone (AQ), nitrene-reactive nitrophenyl azide (NPA), and carbene-reactive phenyl-(trifluoromethyl)diazirine (PTD). This study addresses the selection of photolinker and the adjustment of reaction conditions, such as the concentration of biomolecule applied, and irradiation time. The use of photolinkers (photoactivatable heterobifunctional crosslinkers) is a popular method to attach biomolecules to polymer surfaces. ![]()
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